2. Blood sampling
TRISOMY test can only be performed from the 11th week of pregnancy onwards* and blood samples don´t need to be collected on an empty stomach. Foetal DNA circulates in maternal blood as early as in the first trimester of pregnancy. This foetal DNA can be isolated using a special laboratory procedure based on a 10ml sample of maternal blood. Blood samples are taken by a female nurse at the doctor’s office, whereupon they are sent to the laboratory to be processed and prepared for analysis.
TRISOMY tests are mostly performed between the 11th and the 22nd week of pregnancy. Choosing the earliest/latest date possible is a decision that generally depends on the pregnant woman’s preference and her physician’s recommendation based on the patient’s health condition.
* In compliance with a resolution adopted by the Ethics Committee of the Slovak Ministry of Health, our laboratory only provides test results to patients once they have completed their 12th week of pregnancy. The test result is disclosed and interpreted to the patient by the doctor who referred the patient for the test.
4. Laboratory blood analysis
As soon as the patient’s payment has been identified, the corresponding blood sample is analysed using a special method. The laboratory subsequently sends the result to the patient’s doctor, usually within 5 working days upon receipt of payment.
1. Blood sample processing
As soon as it reaches the laboratory, the blood sample is centrifuged, as a result of which the blood plasma contained in the sample is separated from blood cells. The plasma obtained in this way is subsequently delivered to the Trisomy test, s.r.o. laboratories, where it is processed further.
2. Plasma cleaning
Using a mutli-step treatment method, the plazma sample is cleansed to obtain circulating cell-free DNA, including the DNA that comes from the patient's foetus. Any undesirable proteins and other chemicals are removed in the process.
3. Preparing DNA samples for analysis
For the purposes of the test, the isolated DNA has to be prepared using a special laboratory process that is called “sequencing library preparation”. This procedure takes about 2 days and involves DNA replication because our experts need a much larger quantity of DNA material compared to what they are able to recover from plasma samples.
4. Sample quality control
TRISOMY test is a costly and time-consuming test whose outcome depends on the quality of the prepared samples for analysis. To make sure it was well-prepared in the laboratory, the “sequencing library” must be checked using special equipment before it is subjected to sequencing analysis.
5. Final analysis – sequencing
If the prepared sample has passed our quality control test, it is ready for sequencing analysis. By analysing millions of fragments in the course of several hours, our laboratory staff can determine whether or not the given foetus is affected by any of the chromosomal aberrations monitored.
6. Test outcome
Sequencing results are sent from the Trisomy test, s.r.o. laboratory back to Medirex, where a result sheet is issued for the physician who referred the patient for the test. Respecting the physician's role of a health care provider, the laboratory will not provide any test results directly to the patient.
5. Delivery of test results
(within 5 working days upon receipt of the payment)
The mother-to-be will receive a text message as soon as the sample is delivered to the laboratory, when her payment is cleared, and also when the analysis itself has been completed.
6. Interpretation of test results
Test results can only be provided to the mother-to-be via the doctor who referred her for the screening test. The laboratory cannot provide any test results directly to the patient (respecting the doctor’s position as a health care provider).
Patients’ and Doctors’ Views
I was more than satisfied with the test! I received my test results within 5 days and I knew exactly what to expect. What's more, the test will tell you whether you're expecting a boy or a girl. I recommend the test by all means!